Protective effect of rhodiola rosea extract on alveolar bone loss in diabetic rats / 中国药理学通报

ABSTRACT

Aim: To investigate the protective effect of Rhodiola rosea extract on alveolar bone injury in diabetic osteoporosis rats. Methods: A diabetic rat model was established by intraperitoneal injection of streptozo-tocin 60 mg · kg-1. After successful establishment of the model, the animals were divided into control group, model group and Rhodiola rosea group. Rhodiola rosea extract was administered orally for eight weeks at a dose of 150 mg · kg-1. The levels of blood glucose, serum ALP, Ca, OPG and RANKL were measured, and the ratio of OPG to RANKL was calculated. At the same time, the alveolar bone histopathology was analyzed, and the number of osteoclasts were counted. Results: After treated with Rhodiola rosea extract for eight weeks, the blood glucose, the levels of serum ALP and RANKL in Rhodiola rosea group were significantly lower than those in model group (P < 0. 01). The serum levels of Ca, OPG and the ratio of OPG/RANKL significantly increased in Rhodiola rosea group compared with those of model group (P < 0. 05). Histological examination showed that alveolar bone trabecule in model group were sparse and fractured, bone resorption was serious, and alveolar bone structure was restored after Rhodiola rosea extract administration. The number of osteoclasts in model group increased significantly, and the number of alveolar osteoclasts decreased after Rhodiola rosea extract administration. Conclusions: Rhodiola rosea extract has protective effect on early alveolar bone injury in diabetic rats, and its mechanism is mainly by regulating ALP and OPG/RANKL/RANK system, and accordingly promoting bone formation and inhibiting bone resorption, and balancing bone turnover.