Effect of type A influenza virus on autophagy of lung macrophages and intervention of serum of Maxing Shigan decoction / 中国药理学通报

ABSTRACT

Aim: To study the effect of influenza A virus on autophagy in lung macrophages and the intervention effect of Maxing Shigan decoction. Methods: Influenza A virus infected RAW264. 7 cells as the research object, the experiment set up Maxing Shigan decoction containing serum low, medium and high doses groups, oseltamivir group, 3-MA + influenza virus group, influenza virus group, 3-MA group, rapamycin group and control group. After 12 hours of intervention, the treated cells were examined as follows; (1) the autophagy of each group was detected by laser confocal microscopy; (2) the autophagy and distribution of virus particles were detected by transmission electron microscopy; (3) the expressions of autophagy marker proteins LC3-1 and LC3-II were detected by Western blot. Results: The serum containing Maxing Shigan decoction could inhibit the membrane aggregation of autophagy marker protein LC3 induced by influenza virus in varying degrees, the increase of autophagosome and autophagy lysosome induced by influenza virus, the expression of LC3-II and the ratio of LC3-U to LC3-1 to some extent, and showed a dose-effect relationship (positive correlation). Conclusion Maxing Shigan decoction may regulate the pathogenesis of influenza A virus by inhibiting autophagy induced by influenza A virus.