Protective effect of scutellarin on myocardial ischemia/reperfusion injury and its mechanism / 中国药理学通报

ABSTRACT

Aim To study the protective effect of scutellarin (Scu) on ischemia/reperfusion (I/R) injury in isolated rat hearts and its mechanism. Methods Acute myocardial I/R injury was induced by Lange-ndorff perfusion in isolated rat hearts. SD rats were randomly divided into five groups; blank group, model group, low, medium and high dose group of Scu (0.3, 3, 30 mg • kg-1). The drug experimental group was perfused with K-H solution containing Scu (0. 3, 3, 30 mg • L-1) in vitro perfusion. Myocardial contractile function was recorded; AST, CK and LDH activities in rat myocardium were detected by kit; ICAM-1, IL-ip, IL-6, IL-18 and TNF-α were detected by ELISA; the morphological changes of myocardium were observed by HE staining; and the expressions of IL-1 p, NLRP3 and NF-kB were detected by Western blot. Results Compared with blank group, the contractile function of isolated rat heart in model group significantly decreased, the activities of AST, CK and LDH in myocardium significantly increased, and the contents of inflammatory factors such as ICAM-1, IL-lp, IL-6, IL-18 and TNF-a significantly increased. The expression of IL-1 (3 and NLRP3 and the nuclear translocation of NF-kB significantly increased. Scu significantly improved the above pathological changes. Conclusion The protective effect of Scu on myocardial I/R injury may be related to the inhibition of NF-KB/NLRP3/IL-1 p pathway.