Study on action mechanism of corn silk in treating type II diabetic rats based on urine metabonomics / 中国药理学通报

ABSTRACT

Aim: To observe the interference of corn water decoction on endogenous metabolites in urine of rats with type 2 diabetes mellitus (T2DM), and to investigate the hypoglycemic mechanism of corn silk in metabonomics. Methods: Wistar rats were fed with high-sugar and high-fat diet for four weeks, and a small dose of streptozotocin was intraperitoneally injected to replicate the T2DM model. The modeled rats were divided into model control group and corn silk group. The corn silk group was given water to the corn water decoction(10. 8 g · kg-1) for four weeks. The state of the rats was observed during the administration, and blood glucose levels were monitored every two weeks. After treatment, the fasted 12 h urine samples were collected and the changes analyzed by UPLC/Q-TOF-MS combined with principal component analysis and orthogonal partial least squares-discriminant analysis methods. Results: Maize water decoction could significantly reduce blood glucose levels and improve symptoms in diabetic rats. In the positive and negative ion modes, a total of 12 differential markers were screened. These differential markers include chenodeoxycholic acid, glycocholic acid, argininosuccinic acid, etc. Conclusions: The hypoglycemic mechanism of corn decoction may be related to the metabolism of tricarboxylic acid, biosynthesis of bile acids, metabolism of color amino acid, etc. It also suggests that it may play a protective role in liver and kidney function.