Pyropheophorbide-a methyl ester-mediated photodynamic therapy inhibits migration of human breast cancer MCF-7 cells / 中国药理学通报

ABSTRACT

Aim To investigate the effect of pyropheophorbide-a methyl ester-mediated photodynamic therapy (MPPa-PDT) on migration in human breast cancer MCF-7 cells as well as its possible molecular mechanism. Methods MCF-7 cells were randomly divided into control group, MPPa group, light group and MP- Pa-PDT group. Cells were co-cultured with MPPa at different concenlrations(0, 0. 5, 1,2,4 jimol • L"1) and exposed to light of different energy intensities, 0. 9, 1. 8, 2. 7, 3. 6 J/cm2). Cell viability was measured by CCK-8; production of ROS was detected by DCFH-DA staining; cell migration was mesured by Tr- answell migration assay and wound healing assay; the expressions of E-cadherin, PTEN, Akt, p-Akt were measured by Western blot. Results Compared with control group, MPPa group and light group, MPPa- PDT could significantly inhibit cell viability of MCF-7 cells in an energy-dependent manner. DCFH-DA staining suggested ROS in MPPa-PDT group was higher than that in the other three control groups; Transwell assay and wound healing assay showed cell migration in MPPa-PDT group was significantly inhibited; Western blot analysis indicated that the expression of E-eadhcr- in, PIXN in MPPa-PDT group increased, while p-Akt decreased. Conclusions MPPa-PDT inhibits MCF-7 cells' migration, and PTEN/AKT signal pathway may be involved in the process.