Gypenosides protect acute alcoholic liver injury through Nrf2/nf-kb signaling pathway in mice / 中国药理学通报

ABSTRACT

Aim To investigate the protective effect of gypenosides on acute alcoholic liver injury in mice and its mechanism. Methods The BALB/c mice were divided into five groups, control, alcoholic injury group and three protection groups by different doses of gypenosides. The mice in protection groups were given different doses of gypenosides everyday for one week. After the mice were sacrificed, the peripheral blood and the liver were collected. The pathology of liver tissue sections was observed by HE staining. The ALT, AST, TC and TG were determined by automatic biochemical analyzer. TNF-a and IL-6 mRNA levels were determined by real-time PGR. The content of MDA and GSH, the activities of SOD and CAT and the level of GSH were determined by kits respectively. The protein levels of proteins of Nrf2 and NF-kB signaling pathways were assayed by Western blot. Results Alcohol treatment increased the levels of ALT, AST, TC and TG as well as the expression levels of TNF-a and IL-6. At the same time, alcohol treatment increased the content of MDA and decreased the activities of SOD, CAT and GSH. The alcohol treatment also inhibited the Nrf2 signaling pathway and induced the NF-kB translocation to nuclear. The gypenoside supplementation significantly inhibited all the above changes in a dose-dependent way. Conclusion The gypenoside supplementation shows significant protection on the acute alcoholic liver oxidative injury in mice by regulating Nrf2/NF- kB signaling pathway.