Therapeutic effect of ethanol extract of pterocarya hupehensis skan on collagen-induced arthritis rats and its mechanism / 中国药理学通报

ABSTRACT

Aim To observe the therapeutic effect of Pterocarya hupehensis Skan ethanol extract ( PHSEE ) on CIA model of SL) rats and to explore the mechanism of anti-inflammation and pro-apoptosis. Methods After the CIA model was established, the body weight, paw swelling rate, as well as arthritis index were recorded every 7 days. The rats were continuously fed with PI ISEE or TG for 28 days. The pathological changes of knee joint synovial membrane were observed in each group, while the apoptotic synoviocytes were evaluated by TUNEL assay. The changes of TNF-c∗, IL-lp, ALT, AST, BUN and SCr in the serum were tested by ELISA. And the expressions of NF-kB p65 phosphorylation ( p-p65 ) , cleaved caspase-3, Bcl-2 and Bax in the synovial tissue protein of the knee joint were detected by Western blot. Results Compared with model group, the paw swelling rate and arthritis index decreased in TG and PHSEE-lreated groups with statistical significance ( /' eulic efficacy on CIA rat. The mechanism may be related to down-regulating the expression of inflammatory cytokines and inducing apoptosis of synovial cells. At the same time, the extract can improve impaired liver and kidney function in CIA rats.