Rosmarinic acid analogue-11 inhibited proliferation and migration of human gastric cancer MGC-803 cells via EGFR-JNK pathway / 中国药理学通报

ABSTRACT

Aim: To explore the effects of RAA-11 on the growth and migration of human gastric cancer cells MGC-803 via the EGFR-JNK pathway. Methods: The effect of RAA-11 on the activity of human gastric mucosa cells GES-1 and human gastric cancer cells MGC- 803 was observed by MTT assay. The effect of RAA-11 on the migration in human gastric cancer MGC-803 cells was detected by scratch test. The effect of RAA- 11 on EGFR mRNA expression in human gastric cancer MGC-803 cells was detected by qRT-PCR. The changes of apoptosis-related proteins caspase-3, Bcl-2, Bax as well as pathway proteins EGFR, JNK, and p-JNK expression in human gastric cancer MGC-803 cells were assessed by Western blot. Results: MTT results indicated that RAA-11 significantly inhibited the proliferation of MGC-803 cells in human gastric cancer compared with human gastric mucosa GES-1 cells (P < 0. 01), suggesting that RAA-11 had a selective effect on MGC-803 cells. The scratch result suggested that RAA-11 could suppress the migration of MGC-803 cells. The results of qRT-PCR indicated that RAA-11 decreased the expression level of EGFR mRNA in human gastric cancer MGC-803 cells. Western blot results suggested that RAA-11 up-regulated apoptosisrelated proteins caspase-3 and Bax in human gastric cancer MGC-803 cells, promoted the expression of pathway proteins JNK and p-JNK (P < 0. 01), downregulated the expression of apoptosis-related proteins Bcl-2, and reduced the expression level of pathway protein EGFR (P < 0. 01). Conclusions: RAA-11 can inhibit the proliferation and migration and induce apoptosis of human gastric cancer MGC-803 cells by the EGFR-JNK pathway.