Neuroprotective effect of salidroside on CoCl2 - Induced hypoxia injury in PC12 cells / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12): 530-534, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-857371
ABSTRACT
Aim:
To investigate the neuroprotective effect of salidroside on CoCl2 -induced hypoxia injury in PC 12 cells, and further explore the mechanism of salidroside on inhibiting complement and neuroprotection.Methods:
The model of CoCl2-induced hypoxia injury in PC 12 cells was established. The expressions of Egrl, Egr4, C3 and the activity of caspase-3 were assessed with different concentrations of salidroside treatment. The expressions of Egrl, Egr4 and the activity of caspase-3 were tested with C3ar inhibitor and salidroside treatment. The expressions of Bax, Bcl-xl, C3 and the activity of caspase-3 were determined with Egr4 siRNA and salidroside treatment.Results:
Salidroside significantly reduced the caspase-3 activity and the protein expression of Bax and C3, and improved the expression of Bcl-xl, Egrl, and Egr4 proteins in CoCl2-induced PC12 cells. Salidroside reduced the caspase-3 activity and the expression of Egrl, Egr4 with C3ar inhibitor treatment. With siRNA interference with the expression of Egr4, salidroside reversed the activity of caspase-3, Bax and Bcl-xl, but the effect on C3 was not obvious.Conclusions:
Salidroside has neuroprotective effect on the model of CoCl2 -induced hypoxia injury in PC12 cells, which is related to the inhibition of complement component C3 and the improvement of the expression of Egr4 by salidroside.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2019
Tipo de documento:
Artigo
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