Progressing growth of tumor cell and synthesis of Bip/GRP78 / 中国药理学通报

ABSTRACT

AIM: To explore the relationship between tumor progressing growth and synthesis of Bip/GRP78 in vitro. METHOD: Using tumor cell culture, ion exchange chromatography, SDS-PAGE, specific enzymatic, chemical catalysis, mass spectra and so on, the synthesis of Bip/GRP78 of cells growth in exponential, confluent and post-confluent phases was examined,and compared to normal breast epithelial cells. RESULTS: During the progressing growth, tumor cells' synthesis of Bip/GRP78 exhibited growth situation, cell density and malignant degree-dependent. CONCLUSIONS: During the progressing growth, tumor cells can maintain its homeostasis by synthesizing Bip/GRP78. This synthesis is intensely growth situation. Cell density and malignant degree-dependent. By this synthesis, tumor cell establishs its defensive system. Because increasing investigate results have shown that Bip/GRP78 can decrease the sensitivity of tumor cell to be killed by cytotoxic T lymphocytes, increase its tumorigencity and prevent its apoptosis. So aiming at destruction of the synthesis of Bip/GRP78 may point to a new approaches to the therapy of cancer.