Quantitative analyses of the combined effects of nano red elemental selenium and glucurolactone on forming liver fibrosis induced by CCl4 in rats / 中国药理学通报

ABSTRACT

AIM: To observe the combined effects of nano red elemental selenium (Nse) and glucurolactone (Glu) on forming liver fibrosis in rats, and to search for a low dose of Nse with therapeutic effect. METHODS: According to the weighted modification method, six compound-dose groups and 2 control groups were set. The rats were given (sc) CCl4 for 10 wk, and the administration began at 4 wk. The indices related to acute liver damage were determined 3 wk after the administration, and to chronic liver damage 6 wk after the administration. RESULTS: At the stage of acute liver damage and within the therapeutic duration of 3 wk, Nse should be used at a larger dose in combination with Glu. Theoretical analysis showed that Nse 200 μg·kg-1 alone was available by the weighted modification method. However, in the combination of Nse and Glu for the chronic liver injury, Nse should be given at a low dose. There was a synergism between Nse and Glu, and some compound-dose groups showed the preventive and therapeutic effect, especially in a dose of Nse 100 μg·kg-1 + Glu 60 mg·kg-1. But theoretical analysis exhibited that Nse 100 μg · kg-1 + Glu 60 mg·kg-1 (ig) might be an optimal combination. CONCLUSION: The combination of Nse and Glu has significant effect in prevention and treatment of the live fibrosis in rats, and it can decrease dose of Nse used.