Anti-breast Cancer Effect of Ononin and Its Mechanism in Vitro / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 194-198, 2020.
Article
em Zh
| WPRIM
| ID: wpr-857784
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: To investigate the anti-breast cancer effect of ononin and its mechanism in vitro. METHODS: After treating with different concentration of ononin, the MCF-7 cells viability was determined by MTS, EdU and Hoechst were used to measure the proliferative ability and apoptotic rate of MCF-7, respectively. The invasion and migration of MCF-7 were determined by Transwell assays. The protein expression including PCNA, apoptosis-related proteins (Bax and Bcl-2) and MMP-9 were evaluated by Western-blotting. RESULTS: Ononin inhibited the cells viability of MCF-7 in a dose-and time-dependent manner. The EdU positive cells were decreased, Hoechst positive cells were increased, invasive and migration cells were decreased after treating with ononin in MCF-7. Moreover, ononin up-regulated Bax expression and down-regulated Bcl-2, PCNA as well as MMP-9 expression. CONCLUSION: Ononin could inhibit the MCF-7 cells proliferation, promote cells apoptosis, decrease cells invasion and migration, which may involve the regulative effect on PCNA, Bax /Bcl-2 and MMP-9 expression.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2020
Tipo de documento:
Article