Construction of A30P Mutant α-Synuclein and Its Influence on Protein Degradation Systems in PC12 Cells / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 2071-2075, 2019.
Article
em Zh
| WPRIM
| ID: wpr-857827
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WPRO
ABSTRACT
OBJECTIVE: To explore the effects of human A30P mutant α-synuclein (α-syn) on autophagy lysosome pathway and ubiquitin proteasome system. METHODS: Constructing stable cell lines expressing human A30P mutant α-syn in PC12 cells, and detecting the levels of autophagy related proteins LC3-II and p62 as well as ubiquitinated proteins. Furthermore, the influence of A30P mutant α-syn on cell viability upon normal and stress conditions was conducted by MTT assay. RESULTS: Expressing A30P mutant α-syn could significantly reduce autophagy related protein LC3-II level and increase level of autophagy substrate p62, as well as promote aggregation of ubiquitinated proteins. Otherwise, expressing A30P mutant α-syn did not reduce cell viability under normal conditions. However, cell viability was significantly reduced under treatment with neurotoxin rotenone or serum free condition in A30P mutant α-syn groups compared with empty vector group. CONCLUSION: Over expression of human A30P mutant α-syn could impair the protein degradation system and increase the sensitivity of cells to toxic insults.
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WPRIM
Idioma:
Zh
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2019
Tipo de documento:
Article