Preparation and Characterization of PTX-MWNTs-COOH and Its in Vitro Release / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare and characterize PTX-MWCNTs-COOH complex and study its in vitro release properties. METHODS: The PTX-MWCNTs-COOH complex was prepared by solvent blending method with paclitaxel as drug,phospholipid as surfactant. It was characterized by laser particle size analyzer,transmission electron microscope (TEM) and DSC analysis. The drug loading was determined by filtration membrane together HPLC method and the entrapment efficiency was determined by microcolumn separation together HPLC method. The in vitro release of the PTX-MWCNTs-COOH complex was studied in PBS buffer at pH 7.4(containing 1% Tween-80) with revers dialysis method. RESULTS: The Zeta potential of PTX-MWCNTs-COOH complex was (-24.5±1.01) mV,the drug loading was(50.09±0.02)%,and the entrapment efficiency was (76.80±0.02)%. The cumulative release in vitro was perfect. CONCLUSION: PTX-MWCNTs-COOH showed good physicochemical properties such as high drug loading and entrapment efficiency. The method of reverse dialysis can be used to study the release behavior of PTX-MWCNTs-COOH in vitro.