Application of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis in Identification of Fritillariae Cirrhosae Bulbus / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify 70 samples of Fritillariae Cirrhosae Bulbus from 16 cities such as Beijing, Tianjin, Changchun,Jilin and so on by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis kit.METHODS: The genomic DNA of Fritillariae Cirrhosae Bulbus was extracted by kit method, and PCR reaction and RFLP identification were carried out. The method of the Chinese Pharmacopoeia of 2015 was used for comparison and re-examination.RESULTS: The purity of the genomic DNA of Fritillariae Cirrhosae Bulbus was very good, the OD260/OD280 value was between 1.90-2.1, and the concentration could reach the requirement of PCR. Seventy samples were tested, of which 37 were positive and 33 were counterfeit, and the false rate was 47.1%.CONCLUSION: The purity and concentration of the nucleic acid meet the requirements of PCR and RFLP. The test results of the samples from 16 cities show that the counterfeit rate of Fritillariae Cirrhosae Bulbus is high in the market. Relevant government departments should strengthen the quality management of the traditional Chinese medicine market.