Chemical Constituents of Ethyl Acetate Fraction from Dioscorea bulbifera / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the chemical constituents of ethyl acetate fraction from Dioscorea bulbifera. METHODS: This compounds were isolated and purified with silica gel and Sephadex LH-20 and preparative liquid chromatography. Their structures were determined on the basis of physicochemical properties and spectroscopic analysis. RESULTS: Twenty-four compounds were isolated and identified as ephemeranyhoquinone(1), N-methyl-2-pyrolidinone(2), aurantiamide acetate(3), 3,4-dihydroxybenzoic acid ethyl ester(4), 2, 7-dihydroxy-3, 4-dimethoxyphenanthrene(5), 4, 5-dihydroblumenol A(6), C-veratroylglycol(7), teasperol(8), vomifoliol(9), Z-6, 7-ligustilide(10), 2, 3, 7-trihydroxy-4-methoxyphenanthrene(11), p-hydroxyphenylacetic acid methyl ester(12), 3, 5, 7, 4′-tetrahydroxyflavone(13), 3, 5, 3′-trimethoxyquercetin(14), 3, 4-dihydroxybenzoic acid(15), 3, 5, 7, 3′, 4′-pentahydroxyflavone(16), daucosterol(17), 5, 3 ′, 4′-trihydroxy-3, 7-dimethoxyflavone(18), epicatechin(19), 3, 7-dihydroxy-2, 4-dimethoxy-9, 10-dihydrophenanthrene(20), methyl eucomate(21), catechins(22), 5, 7-dihydroxy-3, 4′-dimethoxyflavone(23), and 3, 4-dihydroxybenzoic acid methyl ester(24). CONCLUSION: Except compounds 11, 14, 15, 18-20, 22 other compounds are isolated from the plant for the first time.