Preparation and performance research of target nmr contrast agent gd-dota-e-2 / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1541-1546, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-858206
ABSTRACT
OBJECTIVE:
To prepare Gd-DOTA-E-2 targeted contrast agent and evaluate its relaxation properties, cytotoxicity and targeting.METHODS:
E-2 was synthesized by F-moc solid phase method, covalently bonded with chelating agent DOTA, meanwhile ultilizing chelating ligand Gd3+ to prepare MRI contrast agent Gd-DOTA-E-2. The structure was characterized by ESI-MS and NMR, and the properties were evaluated by relaxation properties, MTT and mouse MRI contrast assay.RESULTS:
ESI-MS and NMR RESULTS showed that Gd-DOTA-E-2 was synthesized successfully, and its relaxation efficiency(k=9.551 mmol-1•L•s-1) is 1.84 times more efficient than a commercially available contrast agent of Gd-DOTA; MTT RESULTS showed that the toxicity of Gd-DOTA-E-2 cell is lower than that of Gd-DOTA; the experimental RESULTS in MRI angiography showed that Gd-DOTA-E-2 is possessing of targeting and can image clearly.CONCLUSION:
Gd-DOTA-E-2 is a kind of MRI contrast agent with good stability, low cytotoxicity and targeted properties in vivo,which is valuable in research and application.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2018
Tipo de documento:
Artigo
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