Animal Model of Lung Adenoma in C57BL/6J Mice Induced by Urethane / 中国药学杂志

ABSTRACT

OBJECTIVE: To compare and optimize the animal model of lung cancer on C57BL /6J mice by three different experimental methods. METHODS: Female C57BL /6J mice were divided into four groups control group, model group , Ⅱ and III. The mice in model groupreceived 800 mg•kg-1 urethane by intraperitoneal injection twice a week, followed by 5 weeks. The mice in model group Ⅱ were injected intraperitoneally with 1 000 mg•kg-1 urethane in the same way with model group . The mice in model group III received urethane at the dose of 1 000 mg•kg-1 intraperitoneally, every 5 day continuing for 8 weeks. The control group was given equal volume of sodium chloride solutions in the same way as the model group Ⅱ. The main organs of all the groups were taken such as heart, liver, spleen, lung, kidney, brain and thymus at the 28th week, and their organs index was calculated. At the same time the lung tissues were examined to get the amount of adenomas and the diameters, and developed the lung and thymus histopathological examination. The bronchial alveolar lavage fluids were collected to classify the cells of the control and experimental groups. RESULTS: Compared with the control group, all the three experimental groups could induce lung adenomas absolutely, the number of tumors in model groupand III was 6, while tumors in model Ⅱ group was about 2. In the three experimental groups the index of lung increased and the brain, spleen and thymus decreased compared with the control group. The bronchial alveolar lavage fluids analysis showed that the number of lymphocytes and polymorphonuclear leukocytes in the model group are more than that in control group. CONCLUSION: All the three experimental groups could induce lung adenomas absolutely. Compared with model group Ⅱ, the model III and are more stable and homogeneous with the similar results of experiment.