Pharmacokinetic Study of Araloside A in Rats Based on LC-MS/MS Techniques / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a highly sensitive and specific LC-MS/MS method to explore the pharmacokinetic properties of araloside A. METHODS: Araloside A was administered in a dose of 50 mg•kg-1 via gastric in fusion and 5 mg•kg-1 by intravenous injection in rats. Araloside A was analyzed by a validated LC-MS /MS method in plasma after intravenous and intragastric administration. The pharmacokinetic parameters were evaluated by software DAS 3.0. RESULTS: The results of pharmacokinetic study showed that the linear range of araloside A was good in 1.0 - 10 000.0 μg•L-1 (r > 0.994 8). The specificity, precision and accuracy, matrix effect and extraction recovery rate and stability all meet the requirements. The main pharmacokinetic parameters for intragastric administration with araloside A 50 mg•kg-1 and intravenous injection of araloside A 5 mg•kg-1 were as follows t1/2 was (8.65 ± 3.22 ) and (2.00 ± 0.21) h, AUC0-t was (277.14 ± 101.00) and (21 194.59 ± 4 385.13) ng•h•L-1, MRT0-t was (7.88 ± 0.64) and (1.21 ± 0.11) h, Vd/F was (2 229.99 ± 1 013.97) and (0.71 ± 0.20) L•kg-1, CL/F was (149.11 ± 62.28) and (0.24 ± 0.05) L•h-1•kg-1, respectively; ρmax was (32.68 ± 10.74) μg•L-1 for intragastric administration and tmax reached(1.21 ± 0.70) h, oral bioavailability of araloside A was about 0.14%. CONCLUSION: The LC-MS/MS method established is specific and sensitive, and can be successfully applied in basic pharmacokinetic study of araloside A in rat plasma.