Protection of Ginkgo-Diyidamolum Injection on Rat Myocardial Cells Injured by Oxygen-Glucose Deprivation / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the protective effect of ginkgo-diyidamolum injection on rat myocardial cells injured by oxygen- glucose deprivation. METHODS: The model of oxygen-glucose deprivation in rat myocardial cells was established. The cells were randomly divided into five groups normal group, oxygen-glucose deprivation group, different ginkgo-diyidamolum injection dosage groups(0.28, 0.84, 1.40 mg•mL-1). The effects of ginkgo-diyidamolum injection on NO level, malondialdehyde (MDA) level, changes in the concentrations of lactate dehydrogenase (LDH), superoxide dismutase (SOD) activity were evaluated. Cells apoptosis rate was determined by flow cytometry. The gene transcription level of myocardial cell gap junction protein 43 (Cx43) was monitored by RT-PCR. RESULTS: Compared with oxygen-glucose deprivation group, ginkgo-diyidamolum injection could reduce the content of intracellular NO, MDA, LDH and enhance the activity of SOD. Ginkgo-diyidamolum injection high dose and middle dose groups obviously improved the myocardial cell morphology, inhibited cell apoptosis and Cx43 gene in myocardial cells. CONCLUSION: Ginkgodiyidamolum injection has a strong protective effect when myocardial cells injured by oxygen-glucose deprivation, which is related to the antioxidant effect, inhibition of cell apoptosis, and maintaining the role of myocardial gap junction channel.