Determination of Rat Plasma Protein Binding Rate of Mesaconine by UPLC-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an analytical method for determination of mesaconine in rat plasma or dialysate, and study the rat plasma protein binding rate of mesaconine. METHODS: The equilibrium dialysis method was applied to determine the in vitro plasma protein binding rate of mesaconine. A UPLC-MS/MS method was established to determine mesaconine in the intra- and extradialysis samples and then the plasma protein binding rate was calculated. RESULTS: The specificity of the established UPLC-MS/MS method was very good. The calibration curve of mesaconine had good linearity over the range of 0.156 3 - 10 μg•mL-1. The intra-day and inter-day precisions and recovery met the requirements of methodology. The plasma protein binding rates of mesaconine were 18.73%, 20.20%, 26.59%, and 23.68% at the concentrations of 0.2, 0.5, 2.0, and 5.0 μg•mL-1, respectively. CONCLUSION: The method is simple, accurate and sensitive, and can meet the requirement for analysis method of biological samples. Mesaconine has a low rat plasma protein binding rate.