Purification Study of Purple Solanum tuberosum Anthocyanins by AB-8 Macroporous / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the purification process of anthocyanins from purple Solanum tuberosum by AB-8 macroporous resin combined with alcohol precipitation method. METHODS: Using River John Blue genotype purple Solanum tuberosum as raw material, purple Solanum tuberosum anthocyanin crude extract was obtained by leaching method. Then, the purification process of the crude extract was studied by using static adsorption (stirring, loading concentration, loading pH, eluent concentration, eluent pH) and dynamic adsorption (loading flow rate and elution flow rate), respectively, taking adsorption rate, resolution rate and color value as the evaluation indexes. On the basis of the single factor experiment, orthogonal optimization analysis was carried out to get the final optimized process condition. RESULTS: The optimum conditions for the purification of AB-8 macroporous resin were as follows stirring, loading concentration 0.500 mg·mL-1, loading pH 2, ethanol concentration 70% and elution pH 1. The column flow rate was 1 mL·min-1, and the elution flow rate was 1 mL·min-1. Under this condition, the color value of anthocyanin was able to reach 75.40, which was 8.43 times higher than that before purification. CONCLUSION: The purification method has high feasibility and can improve the purity and color value of anthocyanins, which laids a foundation for the market development and application of anthocyanins of purple Solanum tuberosum.