Sensitizing Effect of Diosgenin on the Apoptosis Induced by Mitoxantrone Hydrochloride in MDR Uterine Sarcoma Cell Lines (MES-SA/MX2) / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the sensitizing effect and mechanism of diosgenin on anti-proliferation activity of mitoxantrone hydrochloride on MDR cells. METHODS: Verapamil was used as positive control. MTT method was used to evaluate the proliferation inhibition effect of diosgenin combined with mitoxantrone hydrochloride on MES-SA/MX2 cells. Western blot method (WB) and RT-qPCR were used to evaluate the effect of diosgeninon protein and gene expression of P-gp respectively. The uptake activity of TMRM was measured by flow cytometry(FCM). ATPase activity was determined by kit. RESULTS: Diosgenin can ehhance proliferation inhibition of mitoxantrone hydrochloride in MDR cell lines (MES-SA/MX2). The reversing index was 2.4 times of verapamil. Diosgenin decreased P-gp protein expression by 22.2% (P<0.01), but had marginal effect on P-gp mRNA. Diosgenin increased the fluorescence intensity of TMRM by 14.5%, and did not affect ATPase activity. CONCLUSION: Diosgenin can increase the sensitivity of mitoxantrone hydrochloride by inhibiting P-gp.