Analysis Method of Isomer in Bepotastine Besilate Eye Drops / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1269-1272, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-858647
ABSTRACT
OBJECTIVE:
To establish a method for isomer analysis of bepotastine besilate eye drops and test the stability of isomer in the preparation.METHODS:
The analysis was performed on an ULTRON ES-CD chiral column (6.0 mm×150 mm, 5 μm). The mobile phase was 0.02 mol·L-1 potassium phosphate monobasic-acetonitrile(75∶25) at the flow of 0.8 mL·min-1. The column temperature was maintained at 35℃ and the detection wavelength was set at 225 nm. The injection volume was 10 μL.RESULTS:
The limit of detection and limit of quantification of bepotastine besilate R-isomer were 12 and 48 ng, respectively. The linear range of bepotastine besilate R-isomer was 0.01-0.1 mg·mL-1 and the repeatability was good at high, medium and low concentrations.CONCLUSION:
The method is simple and accurate, which can be used for isomer separation of bepotastine besilate and testing the stability of isomer in bepotastine besilate eye drops.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2017
Tipo de documento:
Artigo
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