Identification of Ophiopogon japonicus from Sichuan by Polymerase Chain Reaction / 中国药学杂志

ABSTRACT

OBJECTIVE: To design specific PCR primers and establish the PCR identification method of Ophiopogon japonicas from Sichuan. METHODS: The gene footprint of Ophiopogon japonicas from Sichuan named CM503 was screened from random amplified polymorphic DNAC(RAPD) amplification. Reclaimed CM503 gene was inserted into T-vector to be cloned and sequenced. One pair of specific primers CM1/CM2 were designed according to the CM503 sequence and applied in specific PCR using the genomic DNA of Ophiopogon japonicas from Sichuan as template. RESULTS: A specific band around 297 bp was detected in Ophiopogon japonicus from Sichuan at 68℃, while nothing appeared for the other varieties. CONCLUSION: The method is convenient, reproducible, and precise, with broad application prospects.