Metabolism study of phillygenin in human liver microsomes by UPLC-Q-TOF-MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a UPLC-Q-TOF-MS method to characterize the metabolites of phillygenin in human liver microsomal incubation system for the first time. METHODS: The chromatography separation was performed on a C18 reversed phase LC column (Phenomenex Kinetex C18, 2.1 mm×100 mm, 2.6 μm). The mobile phase consisted of water-formic acid (1000.1, V/V) and acetonitrile and a gradient elution program was adopted at the flow rate of 400 μL·min-1. The mass spectral analysis was performed in a positive electrospray ionization mode, and the turbo spray temperature was 550℃. The full MS experiment was run with a scan range from m/z100 to m/z 1 000. RESULTS: The possible fragmentation pathways of phillygein were speculated in a positive electrospray ionization mode, and eight metabolites was identified in human liver microsomal incubation system. CONCLUSION: The UPLC-Q-TOF-MS method is very convenient and efficient for detecting phillygein in human liver mirosomes. The developed method is suitable for the metabolism research of phillygein in human liver microsomes, which providing valuable reference for pharmacokinetic study of phillygenin.