Induction and proliferation culture of Adventitious roots of Aconitum pendulum Busch / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the induction and culture system of adventitious roots of Aconitum pendulum Busch. METHODS: By tissue culture, the influences of different explants and plant growth substances on the induction and proliferation of adventitious roots of Aconitum pendulum Busch were investigated. RESULTS: Mixture of MS+2.5 mg·L-1 indole-3-butyric acid (IBA)+0.5 mg·L-1 6-benzylaminopurine (6-BA)+1.0 g·L-1 polyvinyl pyrrolidone (PVP) was the best callus induction medium. Mixture of MS+0.5 mg·L-1 IBA+1.0 g·L-1 PVP was the optimum adventitious root induction medium. Mixture of MS+0.5 mg·L-1 IBA+1.0 g·L-1 PVP was the most suitable adventitious root proliferation medium. CONCLUSION: A culture system is preliminarily established for the induction and proliferation of adventitious roots of Aconitum pendulum, which provides a new way for the resource exploitation and utilization of Aconitum pendulum Busch.