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Simultaneous determination of multiple constituents in scrophulariae radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal ; (24): 1416-1422, 2016.
Artigo em Chinês | WPRIM | ID: wpr-859010
ABSTRACT

OBJECTIVE:

To develop a method for the determination of iridoid, phenylpropanoid glycosides, and organic acids in Scrophulariae Radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS.

METHODS:

The analysis was carried out on a BDS HYPERSIL C18 column (4.6 mm×250 mm, 5 μm) with elution by mobile phase of acetonitrile-water at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 35℃. The target compounds were analyzed by the negative ion multiple reaction monitoring (MRM) mode.

RESULTS:

Twelve multiple constituents showed good linearity (r>0.9994) in the range of the tested concentration. The average recoveries of the 12 components were 99.59%-101.24% with relative standard deviations of 0.93%-1.60%.

CONCLUSION:

The established method is accurate and precise, which provides a reliable and effective technique for the quality evaluation of Scrophulariae Radix.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Pharmaceutical Journal Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Pharmaceutical Journal Ano de publicação: 2016 Tipo de documento: Artigo