Simultaneous determination of multiple constituents in scrophulariae radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1416-1422, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-859010
ABSTRACT
OBJECTIVE:
To develop a method for the determination of iridoid, phenylpropanoid glycosides, and organic acids in Scrophulariae Radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS.METHODS:
The analysis was carried out on a BDS HYPERSIL C18 column (4.6 mm×250 mm, 5 μm) with elution by mobile phase of acetonitrile-water at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 35℃. The target compounds were analyzed by the negative ion multiple reaction monitoring (MRM) mode.RESULTS:
Twelve multiple constituents showed good linearity (r>0.9994) in the range of the tested concentration. The average recoveries of the 12 components were 99.59%-101.24% with relative standard deviations of 0.93%-1.60%.CONCLUSION:
The established method is accurate and precise, which provides a reliable and effective technique for the quality evaluation of Scrophulariae Radix.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2016
Tipo de documento:
Artigo
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