Extraction and purification process of the active ingredient eurycomanone from Eurycoma longifolia Jack / 中国药学杂志

ABSTRACT

OBJECTIVE: To optimize the extraction and purification process of eurycomanone (EN) from Eurycoma longifolia Jack. METHODS: Using single factor test and orthogonal test to screen the best conditions of ethanol concentration, extraction time, amount of solvent and extraction times with the index of transfer rate of EN. Resin model was screened by static adsorption and desorption test, purification process of EN was investigated by single factor test and orthogonal test. RESULTS: The optimal condition of the extracting was 20-fold water, 2 times with the first 2 and 1 h for the second time. Optimal purification process of HPD100 macroporous resin was 70% amount of saturated adsorption sample, 0.25 g·mL-1 of sample liquid concentration, 3BV·h-1 of sample flow rate, eluting using the 30% ethanol, 3 BV·h-1 of elution velocity. CONCLUSION: The optimized process is simple, stable and repeatable, which is suitable for industrial production.