Fingerprint comparative analysis of Cistanche deserticola Y.C. Ma and Cistanche tubulosa (Schrenk) Wight / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the UPLC fingerprint of Cistanche deserticola Y.C. Ma and Cistanche tubulosa (Schrenk) Wight. METHODS: The UPLC fingerprints of 10 batches of C. deserticola and C. tubulosa were determined on an ACQUITY UPLC® BEH C18 column (2.1 mm×50 mm, 1.7 μm) eluted with the mobile phase consisting of 0.2% formic acid solution and acetonitrile in gradient mode at a flow rate of 0.4 mL·min-1, the column temperature was 40℃, and the detection wavelength was set at 240 nm. The chemical attribution of the fingerprint was determined by reference substance comparison method. RESULTS: The common mode of the UPLC fingerprint of C. deserticola and C. tubulosa was set up under the established conditiom. There were 15 common peaks in the fingerprints of 10 samples, five of which were identified. The similarities varied from 0.667 to 0.905 and from 0.249 to 0.991 for Cistanche deserticola Y.C. Ma and Cistanche tubulosa (Schrenk) Wight, respectively. There was significant difference in the fingerprints between C. deserticola and C. tubulosa. CONCLUSION: The method is simple and reliable, which can be readily utilized for distinguishing C. deserticola from C. tubulosa and their quality control.