Simultaneous determination of three flavone constituents in ophiopogonis radix by HPLC method / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an HPLC method for simultaneous determination of methylophiopogonone A, methylophiopogonanone A, and methylophiopogonanone B. METHODS: Comatex C18 column (4.6 mm×50 mm, 5 μm) was used for the HPLC analysis. The mobile phase consisted of acetonitrile and water (5842) and was eluted at the flow rate of 1 mL·min-1. The column temperature was maitained at 30℃. The detection wavelength was set at 280 nm. The injection volume was 15.0 μL. RESULTS: The linear regression equations of methylophiopogonone A, methylophiopogonanone A and methylophiopogonanone B were Y=493321p+31262 (r=0.9999), Y=605744p+40941 (r=0.9999), and Y=586672p+39657 (r=0.9999), respectively. The average recovery rates of the three flavones respectively were 100.59% (RSD=1.51%), 99.27% (RSD=1.28%), and 100.04% (RSD=1.33%). CONCLUSION: The established method for simultaneous determination of flavone constituents in Ophiopogonis Radix is accurate and sensitive, with good repeatability. It can be applied to the quality evaluation of Ophiopogonis Radix.