Preparation, in Vitro and in Vivo evaluation of pyridostigmine bromide-phospholipids complex nanoemulsion / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare pyridostigmine bromide-phospholipids complex nanoemulsion (PPNE) and carry out in vitro and in vivo evaluation. METHODS: The pyridostigmine bromide-phospholipids and PPNE were prepared by solvent evaporation method and pseudo-ternary phase diagram method, respectively. The morphology, particle size, Zeta potential, in vitro release and oral bioavailability of PPNE were evaluated. RESULTS: The PPNE was approximately circular. The particle size and Zeta potential were 26.16 nm and -3.88 mV, respectively. The in vitro release behavior of PPNE was similar to pyridostigmine bromide. The relative oral bioavailability of PPNE was 208.1% to pyridostigmine bromide. CONCLUSION: PPNE was successfully prepared with a simple and repeatable method, which may be a fundamental formulation for further research of pyridostigmine bromide.