Inhibitory effects of phenolic glycoside compound xylocontroside D against Aβ1-42 induced neurotoxicity / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the inhibitory effects of phenolic glycoside compound xylocontroside D on neurotoxicity of primary cultured cortical neurons induced by Aβ1-42. METHODS: The primary cortical neurons and microglia cells derived from rat cerebral tissues were used and neurotoxicity were induced by Aβ1-42. Then the thiazolyl blue tetrazolium bromide test (MTT) was applied for detecting cell survivability; cells were incubated with CH3-H2DCF-DA for evaluating cellular endogenous reactive oxygen species (ROS) level by flow cytometry; fluorescent staining of 8-hydroxy-2-deoxyguanosine (8-OH-DG) was applied for identifying the metabolites of ROS based DNA damage; fluorescent imaging was applied for illustrating the activation of microglia cells and the enzyme-linked immunosorbent assay (ELISA) was applied for analysis of pro-inflammatory cytokines, cyclooxygenase-1 (COX-1), cyclooxygen-ase-2(COX-2), interleukin-1beta (IL-1β) and tumor necrosis factor alpha (TNF-α). RESULTS: Xylocontroside D showed significant inhibitory effects on Aβ1-42 induced neurotoxicity, including cell death, cellular endogenous ROS levels and DNA damage caused by ROS reaction. Moreover, this compound could also prevent the activation of microglia, decrease the production or secretion of proinflammatory cytokines that produced by activated microglia, such as COX-1, COX-2, IL-1β and TNF-α. CONCLUSION: Our results indicated that xylocontroside D was able to inhibit the oxidative stress injury and neuroinflammation of primary cultured cortical neurons induced by Aβ1-42.