Determination of organochlorine pesticide residues in 13 Miao medicines in Guizhou by UAE-SPME-GC method / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a new gas chromatography method coupled with ultrasound-assisted extraction and solid-phase microextraction for rapid analysis of organochlorine pesticide residues (OCPs) in 13 Miao medicines in Guizhou. METHODS: Flos Lonicerae Japonicae was used as an optimization object. Before being analyzed by GC, the sample was extracted by organic solvent and ultrasound, then enriched by SPME. RESULTS: Under the optimized conditions, the linearities of eight kinds of OCPs ranged from 12.5 to 500 ng · g-1, all with good correlation coefficients. The spiked recoveries ranged from 70.4% to 119.8% and relative standard deviations (RSDs) ranged from 8.9% to 14.1%. The detection limits were 1.03-3.45 ng · g-1. CONCLUSION: UAE-SPME-GC method simplifies the preparation steps for traditional Chinese medicines by direct sample enrichment and analysis thus sparing further purification. It is a convenient, environmentally-friendly and rapid method.