Inhibitory effect of evodiamine on neointima hyperplasia of rat carotid arteries with balloon injury / 中国药学杂志

ABSTRACT

OBJECTIVE: To examine the inhibitory effects of evodiamine on vascular neointimal hyperplasia in balloon-injured carotid artery of rats and explore the possible mechanisms. METHODS: Healthy male Sprague Dawley rat vascular neointimal hyperplasia model was made by rubbing the endothelia of common carotid artery with a balloon, and then the rats were randomly divided into sham operation control group, model control group, evodiamine low(40 mg · kg-1) and high(80 mg· kg-1) dose groups. Evodiamine was intragastric administration for 14 consecutive days, and the sham operation or control rats were given with distilled water. After consecutive 14 d, the neointimal hyperplasia degree was observed by histopathological alterations and by calculating the proliferating cell nuclear antigen(PCNA) positive cells expression percentage in the common carotid arter-y. The levels of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) in plasma of rats were measured respectively. The mRNA expressions of PCNA, endothelial nitric oxide synthase(eNOS) and SM α-actin in carotid artery wall were analyzed separately by real time RT-PCR. RESULTS: The neointimal hyperplasia was very serious, as evidenced by thickened neointima and narrowed lumen in carotid artery balloon-injured rats (model control group). Compared with the model control group, evodiamine 40 and 80 mg · kg-1 administration could significantly ameliorate the histopathology of common carotid artery, decrease the positive expression rate of PCNA, but increase the levels of NO, cGMP in plasma of rats, downregulate the PCNA mRNA expression and upregulate mRNA expressions of eNOS and SM α-actin, respectively. CONCLUSION: Evodiamine can depress the vascular neointimal hyperplasia induced by artery endothelia rubbing in rats, the mechanism may be related, at least partly, to the promotion of NO production.