Mechanism of vaccinium vitis procyanidin induced the apoptosis of SHG-44 glioma cells through mitochodrial pathway / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the effects of Vaccinium vitis procyanidin on glioma SHG-44 cells and explore the mechanism of Vaccinium vitis procyanidin induced apoptosis. METHODS: SHG-44 cells were cultured. The rates of inhibition were examined by MTT assay; and the density of SHG-44 cells was observed by inverted microscope; the expression of bax protein was detected by cyto-histoimmunochemistry, bax, bcl-2 and caspase 3 proteins were examined by Western blot assay. RESULTS: The rates of inhibition of SHG-44 cells were increased in 8, 40 and 400 μg · L Vaccinium vitis procyanidin groups, and the density was decreased; the apop-totic rates were increased with dose increased; the expression of bax and caspase 3 proteins was increased in treated groups, but the expression of bcl-2 protein was decreased in treated groups. CONCLUSION: Vaccinium vitis procyanidin could induce the apoptosis in glioma SHG-44 cells through up-regulating the expression of bax protein and down-regulating the expression of bcl-2 protein, and then activated the mitochondrial pathway.