Simultaneous determination of five effective components in Huangdianxiao capsules by UPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a UPLC method for simultaneous determination of puerarin, daidzin, daidzine, palmatine, and berberine in HuangdiAnxiao capsules (HDAXC). METHODS: The chromatographic analyses were carried out on an ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm; Waters Corp., USA) maintained at 30℃. The mobile phase component A was acetonitrile and B was phosphoric acid with gradient elution at 0.2 mL·min-1. The five components were detected by dual wavelength, ie λ1=250 nm, λ2=354 nm. RESULTS: The standard curves of puerarin, daidzin, daidzine, palmatine, and berberine showed good linear relationship in the ranges of 0.022-0.176 μg (r=0.9999), 0.0043-0.0344 μg (r=0.9999), 0.00061-0.0049 μg (r=0.9997), 0.0023-0.0184 μg (r=0.9997), and 0.0086-0.0668 μg (r=0.9996). respectively. The average recoveries were 97.70%, 96.81%, 99.36%, 99.60% and 98.67% with RSDs of less than 2.7% (n=6). CONCLUSION: This method is simple, rapid, accurate, and reproducible, and it can lay basis for the quality evaluation of HDAXC.