Content determination method of crotamiton / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the content determination method of crotamiton. METHODS: The quantitative mass balance method, HPLC external standard method and nuclear magnetic resonance (QNMR) were used to determine the content of crotamiton, respectively.The accuracy of the three methods was evaluated. RESULTS: The contents of crotamiton were 99.2%, 102.9% and 99.1% respectively as determined by the three different methods. CONCLUSION: Because the ultraviolet absorption coefficients of cis- and trans-crotamiton might be different, the current pharmacopoeia method, ie, using integrated peak areas to calculate the content, is questionable. QNMR method can measure the contents of cis- and trans-crotamiton respectively, so it can be a complementary method to establish the reference standard.