Indentification of several kinds of post-translational modifications in recombinant protein pharmaceuticals using MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1726-1730, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-859674
ABSTRACT
OBJECTIVE:
To identify several kinds of post-translational modifications in recombinant protein pharmaceuticals by MS/MS.METHODS:
Firstly the proteins were digested with enzymes, and the obtained peptides were analyzed by UPLC-MS. By MS analysis, peptides with post-translational modifications could be detected, then it was then identified by MS/MS.RESULTS:
Using MS/MS, the disulfide bonds, peptides with similar molecular mass, O-glycosylation site and the type of N-linked oligosaccharides were identified.CONCLUSION:
MS/MS analvsis can make up for the deficiency of enzyme digestion in some extent, and provide more sufficient evidence for the identification of post-translational modifications.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2015
Tipo de documento:
Artigo
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