Research on the HPLC characteristic fingerprints of Rhizoma Pinelliae and processed products / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish and compare the HPLC fingerprints of different parts of Rhizoma Pinelliae (RPI), Rhizoma Pinelliae Praeparatum Cum Alumine (RPA), Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine (RPZ), Rhizoma Pinelliae Praeparatum (RPP) and Jing Pinelliae (JPI) and provide reliable method and scientific basis for their quality control. METHODS: The HPLC fingerprints of water, 75% ethanol and 95% ethanol extracts of Rhizoma Pinelliae and processed products were established and analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A). RESULTS: The chromatograms of water, 75% ethanol and 95% ethanol extracts were generated as the representative standard fingerprints. Thirteen common peaks were obtained in the fingerprints of Rhizoma Pinelliae and Rhizoma Pinelliae Praeparatum Cum Alumine; 15 common peaks were obtained in the fingerprints of Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine; 17 common peaks were obtained in the fingerprints of Rhizoma Pinelliae Praeparatum and Jing Pinelliae. Among the common peaks, 8 characterized peaks were identified as inosine, guanosine, adenosine, succinic acid, ephedrine hydrochloride, liquiritin, glycyrrhizic acid, and 6-Gingerol. Guanosine, adenosine, succinic acid, and ephedrine hydrochloride existed in Rhizoma Pinelliae and processed products. 6-gingerol was only detected in Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine. Liquiritin and glycyrrhizic acid were detected in Rhizoma Pinelliae Praeparatum and Jing Pinelliae. A new peak (peak 8) appeared in the chromatograms of Rhizoma Pinelliae Praeparatum Cum Alumine and Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine; Rhizoma Pinelliae Praeparatum and Jing Pinelliae did not show inosine and had a new peak (peak 11) compared to Rhizoma Pinelliae. CONCLUSION: This study established the HPLC characteristic fingerprints of different parts of Rhizoma Pinelliae and processed products. The method is stable, time-saving, reliable and can identify Rhizoma Pinelliae and its four different processed products, which provides a scientific basis for the quality control of Rhizoma Pinelliae.