Quality evaluation and quality equivalence of Gentiana rigescens Franch. from different populations / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish evaluation methods of the quality and quality equivalence of Gentiana rigescens Franch. from different populations. METHODS: The contents of the active ingredients, gentiopicroside, swertiamarin and sweroside in the roots of Gentiana rigescens Franch., were determined by HPLC, and the HPLC fingerprint was established. The quality and quality e-quivalence were evaluated by One-way ANOVA, cluster analysis and principal component analysis (PCA). RESULTS: The overall quality of Gentiana rigescens Franch. from different populations in Dali Yunnan was better. The content of gentiopicroside in the sample from Weishan population was lower than those in the other populations, while it was more than twice the national standard. There was significant difference (P<0.01) in the contents of gentiopicroside, swertiamarin, and sweroside in the roots of Gentiana rigescens Franch. from different populations. The quality equivalence of Gentiana rigescens Franch. from different populations was analyzed, and 11 common peaks in HPLC fingerprint were selected to evaluate the similarities of the crude drugs. The similarities of the characteristic peaks were all above 0.988. The three characteristic peaks of gentiopicroside, swertiamarin and sweroside which were related to the activity of Gentiana rigescens Franch were identified, and the qualities of different populations were basically equivalent. The total contents of gentiopicroside, swertiamarin and sweroside of Gentiana rigescens Franch. from different populations were similar as shown by cluster analysis and PCA of HPLC fingerprint. CONCLUSION: Jianchuan and Heqing polulations are quality germplasm populations according to the contents of effective components and HPLC fingerprint. The evaluation methods of quality and quality equivalence of Gentiana rigescens Franch. from different populations are established, which would provide the theoretical basis for further filtering of quality germplasm resources of Gentiana rigescens Franch. from wild populations.