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Effect of Schisandra lignans on the apoptosis of SHG-44 glioma neurospheres / 中国药学杂志
Chinese Pharmaceutical Journal ; (24): 113-116, 2014.
Artigo em Chinês | WPRIM | ID: wpr-859867
ABSTRACT

OBJECTIVE:

To explore the mechanism of Schisandra lignans for inducing the apoptosis of human glioma SHG-44 neurospheres.

METHODS:

The effects of Schisandra lignans on the SHG-44 neurospheres proliferation were detected by MTT. The apoptotic rate was analyzed by Annexin-V/PI double staining. The secretion of the bax, bcl-2 and caspase 3 protein was detected by Elisa assay. The expression of bcl-2 protein was examined by Western blot.

RESULTS:

Schisandra lignans could inhibit the proliferation of SHG-44 neurospheres with the concentration of 50,100,200 mg · L-1 in a dose-dependent manner. Schisandra lignans could induce the apoptosis of SHG-44 neurospheres in a dose-dependent manner. The secretion of bcl-2 was decreased, but ratio of bax/bcl-2 was increased, caspase 3 was increased, the expression of bcl-2 protein was down-regulated.

CONCLUSION:

Schisandra lignans could induce the apoptosis of human glioma SHG-44 neurospheres. Its mechanism is correlated with down-regulation of the expression of bcl-2 and up-regulation of the ratio of bax/bcl-2, and then activated caspase 3.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Pharmaceutical Journal Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Pharmaceutical Journal Ano de publicação: 2014 Tipo de documento: Artigo