Effect of Schisandra lignans on the apoptosis of SHG-44 glioma neurospheres / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 113-116, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-859867
ABSTRACT
OBJECTIVE:
To explore the mechanism of Schisandra lignans for inducing the apoptosis of human glioma SHG-44 neurospheres.METHODS:
The effects of Schisandra lignans on the SHG-44 neurospheres proliferation were detected by MTT. The apoptotic rate was analyzed by Annexin-V/PI double staining. The secretion of the bax, bcl-2 and caspase 3 protein was detected by Elisa assay. The expression of bcl-2 protein was examined by Western blot.RESULTS:
Schisandra lignans could inhibit the proliferation of SHG-44 neurospheres with the concentration of 50,100,200 mg · L-1 in a dose-dependent manner. Schisandra lignans could induce the apoptosis of SHG-44 neurospheres in a dose-dependent manner. The secretion of bcl-2 was decreased, but ratio of bax/bcl-2 was increased, caspase 3 was increased, the expression of bcl-2 protein was down-regulated.CONCLUSION:
Schisandra lignans could induce the apoptosis of human glioma SHG-44 neurospheres. Its mechanism is correlated with down-regulation of the expression of bcl-2 and up-regulation of the ratio of bax/bcl-2, and then activated caspase 3.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2014
Tipo de documento:
Artigo
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