Effect protein kinase inhibitor on luteolin glucuronidation / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the effect of protein kinase inhibitor on luteolin glucuronidation. METHODS: LS174T cells were treated with either curcumin or calphostin C of various concentrations. The glucuronidation metabolites of luteolin, catalyzed by the S9 of treated LS174T cells, was measured by HPL C. The expression of UGT1A in treated LS174T cells was determined by realtime PCR and western blot. RESULTS: A rapid down-regulation of luteolin glucuronidation catalyzed by LS174T cells following curcumin and calphostin C treatment was observed. However, there was no effect on the expression of UGT1A in treated LS174T cells. CONCLUSION: The glucuronidation of luteolin can be suppressed by protein kinase inhibitor indirectly. The suppression may be caused by abnormal phosphorylation of UGTs, which are catalyzed by protein kinase.