Sonication Induces Apoptosis in HL-60 Cells / 계명의대학술지
Keimyung Medical Journal
;
: 109-119, 2014.
Artigo
em Coreano
| WPRIM
| ID: wpr-85996
ABSTRACT
To investigate a specific mechanism of apoptosis induced by sonication, we applied 20 kHz ultrasound to leukemia cell line HL-60 with different intensities (0-60 W/cm2) and time durations (0-100 sec). In accordance with previous reports, ultrasound treatment in HL-60 cells induced immediate cell death and delayed cell death which are associated with cell lysis and apoptosis, respectively. Delayed cell death of HL-60 was also detected 5 hours after sonication in our experiment. Detection of caspase activation by Western blot and sub-G1 accumulation by flow cytometry confirmed that apoptosis plays a role in delayed cell death induced by sonication in HL-60 cells. In addition, we found that decrease in lysosomes of HL-60 cells after sonication suggesting lysosomal rupture is involved in the mechanism of cell death induced by sonication.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ruptura
/
Sonicação
/
Terapia por Ultrassom
/
Leucemia
/
Linhagem Celular
/
Western Blotting
/
Ultrassonografia
/
Morte Celular
/
Apoptose
/
Células HL-60
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
Keimyung Medical Journal
Ano de publicação:
2014
Tipo de documento:
Artigo
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