Identification of Clematis species by PCR-SSCP analysis / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a rapid and accurate identification method to distinguish species of Clematis. METHODS: First, the registered ITS sequences of Clematis were seeked in the genbank database and compared with those of three species of Clematis' to confirm the stable specific identification site. Second, specific primers were designed, and PCR-SSCP was used to test Clematis. RESULTS: The fingerprints of the samples existed significant differences, indicating that our method was able to tell apart the different species of Clematis. CONCLUSION: PCR-SSCP has the advantages of high specificity and good reproducibility, therefore, it lays the foundation for the further development of accurate identification of Clematis.