Allozyme variation and genetic diversity of Sophora alopecuroides / 中国药学杂志

ABSTRACT

OBJECTIVE: To assess the allozymic variation and genetic diversity of Sophora alopecuroides populations at biochemical level. METHODS: Vertical slab polyacrylamide gel electrophoresis was adopted to study the genetic difference of 720 samples from 24 populations in Ningxia, Gansu, Qinghai, Xinjiang and Inner Mongolia. Data was analyzed by POPGEN 32, and a cluster diagram was presented by UPGMA. RESULTS: A total of 26 loci in 5 enzyme systems (esterase, EST; malic enzyme, ME; malate dehydrogenase, MDH; peroxidase, PER; shikimate dehydrogenase, SKD) were detected and a high level of genetic diversity was revealed at population level. The percentage of polymorphic loci (P) was 73.08%; the mean expected heterozygosity (He) was 0.337; Shannon's index (I) was 0.517; the coefficient of gene differentiation (FST) among 24 populations was 0.427, and the mean gene flow (Nm) was 0.687, respectively. The genetic identity among the populations ranged from 0.5815 to 0.9261 with the average of 0.7690. The genetic differentiation was found among the populations based on the clustering analysis. CONCLUSION: There is a high level of genetic diversity among different populations, which mainly comes from genetic differentiation. No obvious relationship between the genetic distance and geographical distribution of the 24 populations is identified.