Effect of Evodia rutaecarpa cultivated in Guizhou Yuqing on rabbit thoracic aorta in vitro and its mechanism / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effect of Evodia rutaecarpa cultivated in Guizhou Yuqing on rabbit thoracic aorta in vitro and its possible mechanism. METHODS: Evodia rutaecarpa water decoction at concentrations from 0.1 to 1.6 mg · mL-1 was cumulatively added into organ bath, the isometric tensions of rabbit thoracic aortic rings with intact endothelium or denuded endothelium were recorded, then the thoracic aortic rings were pre-incubated with L-NAME, indometacin and barium chloride respectively to observe the effect of Evodia rutaecarpa. RESULTS: The water decoction of Evodia rutaecarpa cultivated in Guizhou Yuqing could dose-dependently increase the tension of rabbit thoracic aortic rings, and the constrictive effect was stronger on endothelium-intact aortic rings than on endothelium-denuded aortic rings. The contraction induced by Evodia rutaecarpa on endothelium-intact aortic rings was decreased by pre-incubation with L-NAME and indometacin. However, Evodia rutaecarpa had no significant effect on endothelium-intact aortic rings when pre-incubated with barium chloride. CONCLUSION: The water decoction of Evodia rutaecarpa cultivated in Guizhou Yuqing has endothelium-dependent contracting effect on rabbit thoracic aorta, and it may also stimulate the release of NO and PGI2from endothelium cells, which partly compromises its vasoconstrictive effects.