Metabolism study of chrysin by zebrafish / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the metabolism of chrysin by model organism zebrafish for the first time, thus to investigate the reasonability of applying zebrafish in drug phase II metabolism. METHODS: Zebrafish was exposed to chrysin solution for 24 h, and the samples of solution and zebrafish body were analyzed by high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI-MS) method. The quasi-molecular ions of compounds in both negative and positive mode were observed for molecule mass information, and the potential structures of the compounds were identified by studying on the mass spectra and comparing with reference data or standards. RESULTS: In addition to the parent compound, three phase II metabolites were identified, including two glucuroni-dation products and one sulfation product. CONCLUSION: The metabolism of chrysin in zebrafish is highly consistent with that identified by current in vivo and in vitro methods, which indicates that it is reasonable using zebrafish to sutdy phase II metabolism. Zebrafish model has the advantages of using far less amount of compound, lower cost, higher efficiency and simple procedure, which may become a novel organism model for quick predication of metabolism of compounds and enrich the available models greatly.