Spectrum-effect correlation of mimic estrogenic activity of seeds of Cuscuta chinensis / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the relationship between the estrogen-like effect of seeds of Cuscuta chinensis and the fingerprints of the seeds of Cuscuta chinensis from different habitats, thus to provide a basis for the establishment of Chinese medicine quality standards based on the relationship between the fingerprinting and pharmacodynamics. METHODS: The fingerprints of seeds of Cuscuta chinensis samples from different habitats were established by HPLC, and the active ingredients of seeds of Cuscuta chinensis with estrogenlike effect were screened by uterotrophic method and MTT assay, and the spectrum-activity relationship was analyzed by bivariate correlation analysis. RESULTS: Twenty-six peaks of seeds of Cuscuta chinensis Lam. were identified as common peaks by HPLC. It was shown by uterotrophic and MTT assay that the seeds of Cuscuta chinensis from No. 20 habitat could significantly promote the proliferation of MCF-7 cells and the increase of uterus weight compared with the blank control group and the positive control group. It was shown that the estrogenic effect of seeds of Cuscuta chinensis was the combined effect of a variety of chemical constituents. CONCLUSION: There exsists correlation between the fingerprints of the seeds of Cuscuta chinensis from different habitats and their estrogenic activity.