Effect of compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc on the contents of four aconitum alkaloid / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the mechanism of compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. by determining the contents of four aconitine alkaloids (hyacinthin, benzoylaconine, benzoylhypaconine and benzoylmesaconine) in decoction made from Radix Aconiti Lateralis Praeparate alone and the co-decoctions made from the two medicinal herbs by HPLC. METHODS: The separation was performed on a Kromasil C18 column (4.6 mm × 250 mm, 5 μm) with a gradient elution of acetonitrile (A) and 0.2% aqueous acetic acid at pH of 6.20 adjusted with triethylamine (B) (0-15 min 25%-31% A; 15-36 min 31%-35% A; 36-40 min; 35%-25% A). The column temperature was set at 30°C. The flow rate was 1.0 mL · min-1 and the injection volume was 20 μL. The detection wavelength was set at 240 nm. RESULTS: The four components showed baseline separation. The linearity of this method was good (r≤0.999), and the average recoveries were 96.9%-101.9% with RSD<3%. Compared with the decoction made from Radix Aconiti Lateralis Praeparate alone, the contents of hyacinthin and benzoylmesaconine in the co-decoctions made from Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. were increased, the contents of benzoylaconine and benzoylhypaconine were decreased, but the sum of the four aconitine alkaloids was increased. CONCLUSION: The compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. can increase the release of aconitine alkaloids. The feasibility of the compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. is elaborated from chemical point of view.